Plant pigments and photosynthesis lab answers

Sequencing Challenge The steps below are part of the Procedure for this lab activity. If the sucrose solution isn't absolutely cold, the heat of the blender will deactivate the chloroplasts.

Plant pigments and photosynthesis lab answers

Screw the lid onto the cuvette and mix by inverting the cuvette several times. Carotenoids also protect the photosynthetic systems from damaging effects of ultraviolet light. So anything that alters the vapor pressure of the solvents will introduce variability in your results. Table 4. The first report will be on plant pigment chromatography, and the second will be on the light reaction of photosynthesis. What reasons can you give for the differences in the percent transmittance between the live chloroplasts that were incubated in the light and those that were kept in the dark? Sure, you may get away with it times The migration of the pigment relative to the solvent is expressed at the constant Rf. Which spinach leaf pigments did you observe in your chromatography experiments?

I already had familiarity with their Serial Box interface and the probes are very reliable. Use the ribbed edge of the coin to crush the leaf cells.

Chapter 8 lab plant pigments and photosynthesis answer key

The color difference is minimal. Each of the components will migrate at varying rates based on their solubility, size and hydrogen bonding with the paper. Light should not be permitted inside cuvette 2 because it is a control for this experiment. Consider wanting to separate two amino acids, one with a water soluble R group, and one with an aromatic, relatively water insoluble group. We had spectacular chromatograms this way. Add 4 ml of distilled water to cuvette 1. The walls and bottom of cuvette 2 were covered with foil and a foil cap was made for the top. On their graphs, most students used the test tube numbers 1 through 11 on the y-axis to show the percent of transmittance, but some related 11 tube to percent transmittance and adjusted the rest accordingly.

Acids, hydrolytic enzymes, and waste materials are released from broken vacuoles; supplies of metabolites are cut off. One thing I did this year was make my chloroplast suspension too concentrated I assume. So, to optimize the reliability of your system, reduce the volume of the chamber, put pads around the inside that are saturated with the solvent, and put a lid on soon after you insert the chromatogram.

chromatography of photosynthetic pigments

In other words, if you shine your light along a north to south axis, look for florescence along an east to west axis. Just get them very warm, enough to denature the proteins, but not enough to cause coagulation.

It usually helps to soak the spinach in cool or cold water under a light for a few hours. Be sure that the pigment line is on top of the pencil line.

Cover the top of cuvette 1 with Parafilm and invert to mix.

photosynthesis lab quiz

Tightly close the lid.

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(PDF) AP Biology Lab Four: Plant Pigments and Photosynthesis